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Direct enantioselective separation of bevantolol by high‐performance liquid chromatography on normal and reverse cellulose chiral stationary phases
Author(s) -
AboulEnein Hassan Y.,
Serignese Vince
Publication year - 1994
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130080106
Subject(s) - chemistry , enantiomer , chromatography , analyte , cellulose , enantioselective synthesis , resolution (logic) , chiral derivatizing agent , high performance liquid chromatography , chiral column chromatography , organic chemistry , catalysis , artificial intelligence , computer science
A direct, isocratic and simple chromatographic method is described for the enantiomeric separation of bevantolol (BEV) using normal and reverse cellulose tris (3,5‐dimethylphenylcarbamate) chiral stationary phases (CSPs) known as Chiralcel OD and Chiralcel OD‐R, respectively. The effect of various alcohols present in the mobile phase on the separation factor (α) and resolution factor ( R s ) has been studied. The method has been used to determine and identify the BEV enantiomers in human urine after oral administration of racemic BEV. The chiral recognition mechanism(s) between the analyte and these chiral stationary phases is proposed.