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Studies on silica‐bonded monoclonal antibody packing material for separation of recombinant interferon by high performance immunoaffinity chromatography
Author(s) -
Feng Wenke,
Geng Xindu
Publication year - 1993
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130070608
Subject(s) - chemistry , monoclonal antibody , chromatography , recombinant dna , affinity chromatography , silica gel , interferon , antibody , biochemistry , enzyme , virology , biology , immunology , gene
A method for the preparation of a new packing material for high performance liquid chromatography by bonding an anti‐interferon monoclonal antibody to the surface of silica gel is described. The high coupling efficiency and activity of the interferon‐αA‐monoclonal antibody (IFN‐αA‐McAb) column were obtained by activated diol‐silica gel with an activating agent. After purification with this packing material, the specific activity of recombinant human interferon‐αA rose by up to 1.03 × 10 7 IU/mg protein and the purification efficiency was increased by approximately 100 times.