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High performance liquid chromatography of tamoxifen and metabolites in plasma and tissues
Author(s) -
Lim C. K.,
Chow Loretta C. L.,
Yuan ZhiXin,
Smith L. L.
Publication year - 1993
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130070606
Subject(s) - chemistry , chromatography , ammonium acetate , extraction (chemistry) , tamoxifen , methanol , centrifugation , high performance liquid chromatography , detection limit , medicine , organic chemistry , cancer , breast cancer
An isocratic reversed‐phase high performance liquid chromatographic method for the determination of tamoxifen and its metabolites in plasma and tissues is described. Plasma or tissue homogenate was extracted with methanol/dimethyl sulphoxide (4:1 v/v). The supernatant after centrifugation was separated on a BDS‐Hypersil column with methanol/0.5 M ammonium acetate (75:25 v/v) as the mobile phase. The recoveries of tamoxifen added to plasma and liver tissue homogenate by the extraction procedure were 102± 1.6 and 98±2.4% (mean±SD, n = 6), respectively. The solutes were detected at 280 nm with a detection limit of 0.25 μg/mL for tamoxifen.