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Chromatographic determination method for 1‐nitropyrene and its metabolites in biological samples with fluorescence detection after on‐line reduction
Author(s) -
Hayakawa Kazuichi,
Terai Noriko,
Suzuki Kumiko,
Dinning Paul G.,
Yamada Masafumi,
Miyazaki Motoichi
Publication year - 1993
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130070505
Subject(s) - chemistry , chromatography , high performance liquid chromatography , detection limit , zinc , fluorescence , acetonitrile , fluorescence spectroscopy , physics , organic chemistry , quantum mechanics
A high performance liquid chromatographic (HPLC) method with fluorescence detection after on‐line reduction for the determination of 1‐nitropyrene (NP), 1‐nitrosopyrene (NSP), 1‐aminopyrene (AP) and N ‐acetylaminopyrene (AAP) has been developed. The reduction efficiency of NP and NSP on a zinc column was found to be higher than that of an electrochemical reducer. Using a HPLC equipped with a zinc column (4.0 mm i.d. × 10 mm) and an imidazole/HCIO 4 (pH 6.8):acetonitrile mobile phase, detection limits ( S / N = 3) of 20‐30 fmol for NP, NSP and AP and 350 fmol for AAP were obtained. NP, NSP and AP were determined in the incubation mixture of NP and Salmonella typhimurium , YG1021, by this method. Time course studies showed that a large ratio of NP was metabolized in the pre‐incubation step of the Ames test.