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A rapid and sensitive determination of salmon calcitonin in solutions containing bovine serum albumin or gelatin
Author(s) -
Fukuda Terumi,
Yun Arifuku,
Imai Kazuhiro
Publication year - 1993
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130070412
Subject(s) - chemistry , chromatography , salmon calcitonin , bovine serum albumin , derivatization , detection limit , gelatin , gradient elution , elution , serum albumin , linear range , phosphate buffered saline , high performance liquid chromatography , calcitonin , biochemistry , medicine
A sensitive and selective reversed phase high performance liquid chromatographic method was introduced for the determination of salmon calcitonin (sCT) in solutions containing bovine serum albumin or gelatin. The method was based on the sensitive fluorescence detection of sCT by postcolumn derivatization with o ‐phthal(di)aldehyde. The sample was loaded onto a reversed phase column, purified by column switching and separated with linear gradient elution using the ion pair technique. A linear relationship was obtained between the peak height and the amount injected in the concentration range 1–40 ng. The detection limit was 10 ng/mL ( S / N = 3) at a sample injection volume of 100 μL.