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High performance liquid chromatographic determination of individual bile acids in serum for automatic diagnosis of various liver and biliary diseases
Author(s) -
Zhao R. H.,
Li B. Y.,
Chen N.,
Zhang Y. K.,
Wang Z. Y.,
Lu P. C.
Publication year - 1993
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130070307
Subject(s) - chromatography , chemistry , ternary operation , high performance liquid chromatography , computer science , programming language
A reversed phase high performance liquid chromatographic method for the high sensitivity determination of individual bile acids in serum using a C18 column with a ternary solvent system combined with fluorometric techniques using immobilized enzymes is described. A computer‐assisted diagnosis system using pattern recognition was developed to assist the clinical diagnosis of various liver and biliary diseases. A total consistency rate of 95% can be reached using this system.