High performance liquid chromatographic determination of individual bile acids in serum for automatic diagnosis of various liver and biliary diseases | Zendy

Zhao R. H. | Zendy; Li B. Y. | Zendy; Chen N. | Zendy; Zhang Y. K. | Zendy; Wang Z. Y. | Zendy; Lu P. C. | Zendy

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High performance liquid chromatographic determination of individual bile acids in serum for automatic diagnosis of various liver and biliary diseases

Author(s) -

Zhao R. H.,

Li B. Y.,

Chen N.,

Zhang Y. K.,

Wang Z. Y.,

Lu P. C.

Publication year - 1993

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.1130070307

Subject(s) - chromatography , chemistry , ternary operation , high performance liquid chromatography , computer science , programming language

A reversed phase high performance liquid chromatographic method for the high sensitivity determination of individual bile acids in serum using a C18 column with a ternary solvent system combined with fluorometric techniques using immobilized enzymes is described. A computer‐assisted diagnosis system using pattern recognition was developed to assist the clinical diagnosis of various liver and biliary diseases. A total consistency rate of 95% can be reached using this system.

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