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A high performance liquid chromatographic procedure for the simultaneous determination of norfloxacin and furprofen in rat plasma
Author(s) -
Carlucci Giuseppe,
Mazzeo Pietro,
Palumbo Giancarlo
Publication year - 1993
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130070303
Subject(s) - chemistry , chromatography , norfloxacin , dichloromethane , phosphate buffered saline , extraction (chemistry) , pharmacokinetics , high performance liquid chromatography , acetonitrile , absorption (acoustics) , antibiotics , ciprofloxacin , pharmacology , solvent , organic chemistry , acoustics , medicine , biochemistry , physics
A rapid and simple high performance liquid chromatographic analytical method is described for the simultaneous determination of norfloxacin and furprofen in rat plasma. Following dichloromethane extraction, the solution was chromatographed in a Vydac anion exchange column using a mobile phase of 0.05 M phosphate buffer (pH=7.0):acetonitrile (80:20, v/v) at a flow‐rate of 1.8 mL/min. The drugs were detected by UV absorption at 278 nm. The total chromatographic analysis time was 10 min. The response was linear, 0.1‐5.0 μg/mL for norfloxacin and 0.1‐3.0 μg/mL for furprofen, respectively. This method is useful for pharmacokinetic studies of these compounds and will facilitate detailed investigations into the interactions between quinolones and furprofen.