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High performance liquid chromatographic analyses of compounds related to nucleic acids by 3‐(1‐naphthoylamino)‐ and 3‐(1‐anthroylamino)‐propyl‐modified silica gel packing materials
Author(s) -
Akiyama Shuzo,
Nakashima Kenichiro,
Yamada Kyoko,
Kuroda Naotaka
Publication year - 1993
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130070213
Subject(s) - chemistry , chromatography , silica gel , nucleic acid , phosphate buffered saline , elution , buffer (optical fiber) , phosphate , aqueous solution , organic chemistry , biochemistry , telecommunications , computer science
The development of column packing materials was tried as a contribution to studies related to the further modification of 3‐aminopropyl‐modified silica gel. Consequently, we prepared two types of gel, 3‐(1‐naphthoylamino)‐ and 3‐(1‐anthroylamino)‐propyl‐modified silica gels (NAPS and AAPS). Since NAPS and AAPS, each having an aromatic ring, were expected to behave in a separation mode by a π‐π or hydrophobic interaction, their application to the analyses of the compounds related to nucleic acids was carried out. Adenosine nucleotides were consequently separated by the gels in a single analysis by a single buffer elution with UV detection. NAPS could be also successfully used for the measurement of ATPase activity in fish meat ( Sillago japonica ). The mobile phase consistently used in the analysis was an aqueous phosphate buffer.