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Free‐solution capillary electrophoretic separation of small hydrophilic peptides
Author(s) -
Zhang Y. K.,
Chen N.,
Wang L.
Publication year - 1993
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130070205
Subject(s) - chemistry , chromatography , capillary electrophoresis , electrophoresis , glycine , capillary action , phosphate buffered saline , buffer (optical fiber) , phase (matter) , amino acid , biochemistry , organic chemistry , telecommunications , materials science , computer science , composite material
Small hydrophilic peptides differing in only one glycine amino acid group have been successfully separated by free‐solution capillary electrophoresis (FSCE) using phosphate buffer at acid pH. The effects of column temperature and operating current on the migration times of these small peptides have been investigated. The results from FSCE have been compared with those from reversed phase high performance liquid chromatography.