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Determination of 5,10,15,20‐ tetra‐ ( m ‐hydroxyphenyl)chlorin in human plasma by high performance liquid chromatography
Author(s) -
Wang Qiang,
Ris HansBeat,
Altermatt H. J.,
Reynolds Brenda,
Stewart J. C. M.,
Bonnett Raymond,
Lim Chang Kee
Publication year - 1993
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130070112
Subject(s) - chemistry , chromatography , trifluoroacetic acid , high performance liquid chromatography , detection limit , chlorin , acetonitrile , tetra , methanol , extraction (chemistry) , photodynamic therapy , organic chemistry , medicinal chemistry
A high performance liquid chromatographic method for the determination of the photodynamic chemotherapeutic agent 5,10,15,20‐tetra( m ‐hydroxyphenyl)chlorin (m‐THPC) in human plasma following intravenous infusion is described. The procedure involves extraction of the drug in plasma with methanol/dimethyl sulphoxide (4:1 v/v) containing 5,10,15,20‐tetra( p ‐hydroxyphenyl)clorin as the internal standard and separation on a C18 reversed phase column with acetonitrile:0.1% trifluoroacetic acid (77:23 v/v) as the mobile phase. The drug was detected specifically and sensitively at its absorption maximum of 423 nm with a detection limit of 15 ng/mL (signal‐to‐noise ratio of 5). The intra‐ and inter‐assay coefficients of variation (CV) on analysis of a plasma spiked with m‐THPC (1 μg/mL) were 2.3 and 3.4% ( n =6), respectively.