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High performance liquid chromatographic determination of azelastine and desmethylazelastine in guinea pig plasma and lung tissue
Author(s) -
Langevin C. N.,
Pivonka J.,
Wichmann J. K.,
Kucharczyk N.,
Sofia R. D.
Publication year - 1993
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130070103
Subject(s) - azelastine , chemistry , chromatography , extraction (chemistry) , high performance liquid chromatography , guinea pig , pharmacology , medicine , endocrinology
Analytical methods have been developed for the simultaneous quantitation of azelastine (AZ) and desmethylazelastine (DAZ) in guinea pig plasma and lung tissue. The methods require a 1.00 mL plasma sample and a minimum 0.100 g lung sample. Both methods employ liquid/liquid organic extraction and back‐extraction into dilute acid. Quantitation is performed by high performance liquid chromatography on a 2X250 mm 5 μm Hypersil TM CPS column using fluroescence detection. The linear quantitative ranges for AZ.HCI and DAZ.HBr in plasma are 0.156–160 ng/mL and 0.313–160 ng/mL, respectively. The linear quantitative ranges for AZ.HCI and DAZ.HBr in lung tissue are 0.039–20 μg/g for both.