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Robotic solid phase extraction and high performance liquid chromatographic analysis of ranitidine in serum or plasma
Author(s) -
Lloyd Thomas L.,
Perschy Teresa Benedetti,
Gooding Ann E.,
Tomlinson Julie J.
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060613
Subject(s) - chromatography , chemistry , extraction (chemistry) , high performance liquid chromatography , solid phase extraction , ranitidine , sample preparation , accuracy and precision , plasma , mathematics , medicine , statistics , physics , quantum mechanics , gastroenterology
A fully automated assay for the analysis of ranitidine in serum and plasma, with and without an internal standard, was validated. It utilizes robotic solid phase extraction with on‐line high performance liquid chromatographic (HPLC) analysis. The ruggedness of the assay was demonstrated over a three‐year period. A Zymark Py Technology II® robotic system was used for serial processing from initial aspiration of samples from original collection containers, to final direct injection onto the on‐line HPLC system. Automated serial processing with on‐line analysis provided uniform sample history and increased productivity by freeing the chemist to analyse data and perform other tasks. The solid phase extraction efficiency was 94% throughout the assay range of 10–250 ng/mL. The coefficients of variation for within‐ and between‐day quality control samples ranged from 1 to 6% and 1 to 5%, respectively. Mean accuracy for between‐day standards and quality control results ranged from 97 to 102% of the respective theoretical concentrations.