Premium
A Selective HPLC/RIA for dexamethasone and its prodrug dexamethasone‐21‐sulphobenzoate sodium in biological fluids
Author(s) -
Hochhaus Günther,
Hochhaus Renate,
Werber Gisela,
Derendorf Hartmut,
Möllmann Helmut
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060607
Subject(s) - chemistry , dexamethasone , chromatography , prodrug , high performance liquid chromatography , pharmacology , dexamethasone sodium phosphate , sodium , biochemistry , medicine , organic chemistry
A combined high performance liquid chromatography/radioimmunoassay procedure is described for the simultaneous determination of dexamethasone (DEX) and its prodrug dexamethasone‐21‐sulphobenzoate sodium (DSS) in plasma. After precipitation of the plasma proteins by acetonitrile, the protein‐free supernatant was injected onto a C 18 reversed phase liquid chromatographic system and DSS‐ and DEX‐containing fractions were collected. Hydrolysis of DSS by 0.01 N NaOH, followed by fractions extraction of both hydrolysed DSS and DEX fractions with ethyl acetate allowed the use of a dexamethasone‐specific radioimmunoassay for the selective determination of both compounds. The method is accurate and reproducible (intraday variability for DSS and DEX<6%, interday variability for DEX 14%), allowing quantification of DEX and DSS as low as 0.3 ng/mL and 0.7 ng/mL, respectively.