Premium
Detection of gangliosides with the fluorochrome NBD dihexadecylamine and its application for preparative high performance thin layer chromatography
Author(s) -
Müthing Johannes,
Unland Frank
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060505
Subject(s) - chemistry , chromatography , high performance thin layer chromatography , thin layer chromatography , fluorescence , chloroform , silica gel , high performance liquid chromatography , physics , quantum mechanics
A new method for the detection of gangliosides based on the lipophilic fluorescence agent 4‐( N , N ‐dihexadecyl)amino‐7‐nitrobenz‐2‐oxa‐1,3‐diazole (NBD dihexadecylamine) and its application for preparative high performance thin layer chromatography is described. Brain gangliosides were chromatographed on silica gel coated thin layer plates and located with non‐destructive fluorochrome under longwave ultraviolet light. The fluorescent zones were scraped off and the gangliosides were extracted with a mixture of chloroform/methanol/water (30/60/8; v/v/v). The gangliosides were separated from uncharged NBD dihexadecylamine by anion exchange chromatography and impurities were removed by Iatrobeads chromatography. The method described offers a simple and succesful preparative thin layer chromatographic strategy to obtain pure gangliosides in microgram and milligram quantities.