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Liquid chromatographic determination of hyoscine (scopolamine) in urine using solid phase extraction
Author(s) -
Whelpton Robin,
Hurst Peter R.,
Metcalfe Roger F.,
Saunders Sally A.
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060410
Subject(s) - chromatography , chemistry , urine , metabolite , extraction (chemistry) , scopolamine , detection limit , solid phase extraction , coulometry , high performance liquid chromatography , pharmacology , biochemistry , medicine , electrode , electrochemistry
A sensitive method for the determination of hyoscine (scopolamine) in urine is described. After concentration and “clean‐up” on C 18 and CN solid phase extraction columns, hyoscine was quantified by high performance liquid chromatography with coulometric detection (oxidation at +0.9 V). The limit of detection was 5 ng per sample and the precision for 5 mL samples containing 2 ng/mL was 12.3%. The method was applied to urine samples collected from 12 volunteers wearing Scopoderm TTS patches. The mean excretion rate of unmetabolized hyoscine was 0.45 μg/h and 87% of the total hyoscine was present as conjugates. Apohyoscine (aposcopolamine) was identified as a urinary metabolite. The significance of this with regard to hyoscine assays is discussed.