Premium
TLC separation of certain tetracycline and amino glycopeptide antibiotics
Author(s) -
Bhushan R.,
Ali Imran
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060409
Subject(s) - chemistry , silica gel , chromatography , tetracycline , formic acid , glycopeptide , tetracycline antibiotics , antibiotics , acetic acid , amino acid , organic chemistry , biochemistry
The separation of tetracycline and amino glycopeptide antibiotics was achieved on silica gel thin layers. Tetracycline antibiotics were resolved on a Co +2 (1.0%) impregnated silica gel layer using ethanol:acetic acid: water (10:6:6, v/v/v) as the mobile phase. Amino glycopeptide antibiotics were separated on an untreated silica gel layer using the mobile phase n ‐butanol: formic acid: water (6:5:7, v/v/v). The spots of these antibiotics were located by exposing the chromatoplate to iodine vapours.