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High performance liquid chromatography with chemiluminescence detection of methamphetamine and its related compounds using 4‐( N , N ‐dimethylaminosulphonyl)‐7‐fluoro‐2,1,3‐benzoxadiazole
Author(s) -
Nakashima Kenichiro,
Suetsugu Keiko,
Yoshida Kazunori,
Akiyama Shuzo,
Uzu Sonoko,
Imai Kazuhiro
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060311
Subject(s) - chemistry , chromatography , chemiluminescence , peroxyoxalate , reagent , detection limit , hydrogen peroxide , high performance liquid chromatography , elution , acetonitrile , oxalate , urine , organic chemistry , biochemistry
A high performance liquid chromatographic assay of methamphetamine (MP) and its related compounds, i.e. ephedrine (EP), norephedrine (NE), p ‐hydroxymethamphetamine ( p ‐HMP), p ‐hydroxyamphetamine ( p ‐HAP) and amphetamine (AP), with peroxyoxalate chemiluminescence detection has been developed. 4‐( N , N ‐Dimethylaminosulphonyl)‐7‐fluoro‐2,1,3‐benzoxadiazole (DBD‐F) was used as a fluorescent labelling reagent. A mixture of hydrogen peroxide and bis[4‐nitro‐2(3,6,9‐trioxadecyloxycarbonyl)phenyl] oxalate in acetonitrile was used as a postcolumn chemiluminogenic reagent. DBD derivatives of MP and its related compounds were separated by a gradient elution with acetonitrile and 0.01 M imidazole buffer (pH 7.0) within 65 min. The detection limits ( S / N = 3) for the proposed method for MP, AP, EP, NE, p ‐HMP and p ‐HAP were 27, 100, 40, 133, 25 and 133 fmol on column, respectively. The recoveries of these compounds with normal urine samples were 87.4–106.4%. The method was successfully applied to the assay of MP and its metabolites in urine samples from MP addicts. A good linear correlation for the resulted amounts of MP or AP between the proposed method and gas chromatography was obtained ( r = 0.993 for MP or 0.991 for AP).