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Determination of panaxadiol and panaxatriol in ginseng and its preparations by capillary supercritical fluid chromatography (SFC)
Author(s) -
Yunhua Li,
Xiulu Li,
Lan Hong,
Jinyao Liu,
MeiYi Zhang
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060209
Subject(s) - chemistry , chromatography , ginseng , supercritical fluid chromatography , supercritical fluid , methyltestosterone , ethanol , adsorption , capillary action , cyclohexane , hydrolysis , column chromatography , organic chemistry , medicine , materials science , alternative medicine , pathology , composite material
Capillary supercritical fluid chromatographic (SFC) method has been developed for the determination of panaxadiol and panaxatriol in ginseng and its preparations. 0.1 g ginseng or an appropriate amount of its preparations was hydrolysed by 15% H 2 SO 4 in an ethanol: water (1:1 v/v) solution for 4 h followed by 15% NaOH for 0.5 h. The mixture was extracted by cyclohexane. The cyclohexane extracts were purified by a partition column and concentrated by an adsorption column and then analysed by SFC. Methyltestosterone was used as the internal standard.