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The combination of normal phase with reversed phase high performance liquid chromatography for the analysis of asparagine‐linked neutral oligosaccharides labelled with p ‐aminobenzoic ethyl ester
Author(s) -
Matsuura Fumito,
Ohta Masaya,
Murakami Koichi,
Hirano Kaoru,
Sweeley Charles C.
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060207
Subject(s) - chemistry , chromatography , amide , asparagine , oligosaccharide , elution , glycoprotein , high performance liquid chromatography , phase (matter) , organic chemistry , amino acid , biochemistry
We have developed a novel approach for the analysis of asparagine‐linked neutral oligosaccharides derived from glycoproteins. The oligosaccharides are labelled with p ‐aminobenzoic ethyl ester and the derivatives are separated on two high performance liquid chromatographic columns, one containing amide‐silica and the other containing octadecyl‐silica. The elution positions of 39 different ABEE‐oligosaccharides on the two columns were plotted on a two‐dimensional map. Unique non‐overlapping positions of these oligosaccharides demonstrate that this technology would be useful for the identification of Asn‐linked oligosaccharides at high sensitivity.