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A sensitive and specific high performance liquid chromatographic assay for imidazole dipeptides and 3‐methylhistidine in human muscle biopsies, serum and urine
Author(s) -
Teahon K.,
Rideout J. M.
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060106
Subject(s) - chemistry , chromatography , urine , imidazole , high performance liquid chromatography , biochemistry
A sensitive and specific high performance liquid chromatographic method is described for measuring imidazole dipeptides and 3‐methylhistidine in human muscle biopsies, serum and urine. Muscle extract, serum or urine was reacted with o ‐phthaldialdehyde and the derivatives were separated by reversed phase chromatography with column swithing and fluorescence detection.