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Determination of 10α‐methoxy‐9,10‐dihydrolysergol, a nicergoline metabolite, in human urine by high performance liquid chromatography
Author(s) -
Sioufi A.,
Sandrenan N.,
Godbillon J.
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060104
Subject(s) - metabolite , chemistry , chromatography , urine , dichloromethane , high performance liquid chromatography , acetonitrile , solvent , biochemistry
A specific method for the determination of 10α‐methoxy‐9,10‐dihydrolysergol, a nicergoline metabolite (metabolite 2), in urine is described. Metabolite 2 was well separated from the urine components on a reversed phase column, Hypersil ODS 5 μm, using an acetonitrile: pH 3.5 phosphate buffer (40:60, v/v) as the mobile phase at a flow rate of 1 mL/min. UV detection was set up at 220 nm. After addition of a known amount of lysergamide as the internal standard, the compounds were extracted from alkalysed urine on a pre‐packed glass column (Extrelut 1) with dichloromethane. With 0.5 mL urine, concentrations down to 0.56 μmol/L could be determined.