Premium
High performance liquid chromatography of spermidine and spermine using a postcolumn reactor of immobilized polyamine oxidase ( Aspergillus terreus ) Followed by Electrochemical Detection
Author(s) -
Watanabe Noriyuki
Publication year - 1992
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130060102
Subject(s) - aspergillus terreus , chemistry , spermidine , polyamine oxidase , spermine , chromatography , polyamine , detection limit , high performance liquid chromatography , linear range , biochemistry , enzyme
A highly sensitive and selective method for the determination of spermidine and spermine has been developed. A polyamine oxidase ( Aspergillus terreus ) immobilized column was used as a postcolumn reactor. The detection limit was 0.2 pmol/injection for both spermidine and spermine with a linear range of three orders of magnitude.