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High performance liquid chromatographic analysis of tetroxoprim and sulphadiazine in serum and urine
Author(s) -
Alkaysi Hanan N.,
Salem Mutaz Sheikh,
Badwan Adnan A.
Publication year - 1991
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130050608
Subject(s) - chemistry , chromatography , perchloric acid , urine , protein precipitation , acetonitrile , benzoic acid , quantitative analysis (chemistry) , high performance liquid chromatography , methanol , ethyl acetate , biochemistry , organic chemistry
Quantitative determination of tetroxoprim and sulphadiazine in serum and urine was performed using reversed phase high performance liquid chromatography. Protein precipitation using 10% perchloric acid was utilized for purification of serum samples while urine samples were diluted prior to analysis. The mobile phase consisted of triethylammonium acetate buffer (85%), acetonitrile (12%) and methanol (3%), with a final pH of 4.2. The eluent was monitored at 280 nm. Benzoic acid was used as an internal standard. Standardization, validation and application of the method is described.