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A high performance liquid chromatographic method for the quantitation of flecainide in plasma
Author(s) -
Broly F.,
Marecaux P.,
Houdret N.,
Lhermitte M.,
Houssin R.
Publication year - 1991
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130050604
Subject(s) - chromatography , chemistry , detection limit , phosphate buffered saline , chromatographic separation , high performance liquid chromatography , acetonitrile , quantitative analysis (chemistry)
A routine high performance liquid chromatographic method for the rapid determination of flecaïnide (Flecaine®), using a novel internal standard, N ‐methylflecaïnide, has been developed. After deproteinization of spiked samples, flecaïnide was totally recovered at neutral pH. Flecaïnide and the internal standard were separated on a reversed phase XL 3 μm ODS column using 10 mM phosphate buffer, pH 3.0: acetonitrile (70:30) as mobile phase, in less than 10 min. With spectrofluorometric detection, the limit of quantitation for flecaïnide was 10 n/mL. Intra‐ and inter‐assay precision variations were 0.24% and 1.4%.