A high performance liquid chromatographic method for the quantitation of flecainide in plasma | Zendy

Broly F. | Zendy; Marecaux P. | Zendy; Houdret N. | Zendy; Lhermitte M. | Zendy; Houssin R. | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

A high performance liquid chromatographic method for the quantitation of flecainide in plasma

Author(s) -

Broly F.,

Marecaux P.,

Houdret N.,

Lhermitte M.,

Houssin R.

Publication year - 1991

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.1130050604

Subject(s) - chromatography , chemistry , detection limit , phosphate buffered saline , chromatographic separation , high performance liquid chromatography , acetonitrile , quantitative analysis (chemistry)

A routine high performance liquid chromatographic method for the rapid determination of flecaïnide (Flecaine®), using a novel internal standard, N ‐methylflecaïnide, has been developed. After deproteinization of spiked samples, flecaïnide was totally recovered at neutral pH. Flecaïnide and the internal standard were separated on a reversed phase XL 3 μm ODS column using 10 mM phosphate buffer, pH 3.0: acetonitrile (70:30) as mobile phase, in less than 10 min. With spectrofluorometric detection, the limit of quantitation for flecaïnide was 10 n/mL. Intra‐ and inter‐assay precision variations were 0.24% and 1.4%.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research