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Application of photodiode array detection and fast atom bombardment mass spectrometry for the identification of the arginine residue in neuropeptides
Author(s) -
Silberring Jerzy,
Nyberg Fred
Publication year - 1991
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130050603
Subject(s) - chemistry , mass spectrometry , chromatography , fast atom bombardment , derivatization , arginine , photodiode , peptide , absorbance , residue (chemistry) , molecule , amino acid , chromogenic , analytical chemistry (journal) , biochemistry , organic chemistry , physics , quantum mechanics
Chemical derivatization by phenylgyoxal (PGX) was applied to the identification of arginine in the neuropeptides dynorphin A (1–6) and substance P. The obtained products were separated on a short reversed phase C 18 column and analysed on‐line with the photodiode array UV technique. The selective attachment of a chromogenic molecule into the arginine residue resulted in significant change in the absorbance spectra around 250 nm, depending on the number of PGX molecules attracted. Further analysis employed fast atom bombardment mass spectrometry (FAB MS) and C‐terminal sequencing for detailed verification of the derivatives formed during modification with PGX. The results clearly demonstrated that the photodiode array technique, when combined with chemical modification of certain amino acids, provides new possibilities for the analysis of peptide structures.