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Determination of short‐chain fatty acids in equine caecal liquor by ion exchange high performance liquid chromatography after solid phase extraction
Author(s) -
Horspool L. J. I.,
McKellar Q. A.
Publication year - 1991
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130050505
Subject(s) - chemistry , chromatography , extraction (chemistry) , ion exchange , solid phase extraction , ion chromatography , phase (matter) , ion , organic chemistry
A high performance liquid chromatography (HPLC) method was developed for the determination of seven short‐chain fatty acids in equine caecal liquor. Samples were cleaned up on a Sep‐pak (C18) cartridge, and the analyte was eluted from the extraction cartridge and filtered through a 0.45 μm cellulose nitrate filter. The analyte was chromatographed by ion exchange HPLC. Detection was by UV at 210 nm. Recovery from phosphate buffer (0.05 M, pH 7.0) and equine caecal liquor was 76.95% (lactic), 76.76% (acetic), 84.40% (propionic), 89.35% (isobutyric), 88.73% (butyric), 80.33% (isovaleric) and 72.61% (valeric). The limit of detection of the short‐chain fatty acids in phosphate buffer was 0.00006 M (lactic), 0.0001 M (acetic), 0.0002 M (propionic), 0.0001 M (isobutyric), 0.0002 M (butyric), 0.0002 M (isovaleric) and 0.0003 M (valeric). The specificity and sensitivity of this method was sufficiently high to allow the characterization of the pattern of these short‐chain fatty acids in equine caecal liquor following intravenous administration of oxytetracycline at the recommended dose rate in a pony.