Premium
Medium‐ and long‐chain 3‐hydroxymonocarboxylic acids: Analysis by gas chromatography combined with mass spectrometry
Author(s) -
Dorland L.,
Ketting D.,
Bruinvis L.,
Duran M.
Publication year - 1991
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130050405
Subject(s) - chemistry , chromatography , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography
Medium‐ and long‐chain 3‐hydroxymonocarboxylic acids represent intermediates in the β‐oxidation of fatty acids: they accumulate in the plasma of patients with an inherited deficiency of long‐chain 3‐hydroxyacylcoenzyme A dehydrogenase. 3‐Hydroxy acids with chain lengths varying from 6 to 16 were synthesized by a Reformatzky reaction. Capillary gas chromatography of the pertrimethylsilyl derivatives was performed on a CP‐Sil 19 CB column, coupled to a quadrupole mass spectrometer in the electron impact mode. Calculation of the retention indices showed that the separation of the 3‐hydroxy acids from the homologous straight‐chain fatty acids may be troublesome, stressing the need for mass spectrometric identification.