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High performance liquid chromatography as a tool in the definition of abnormalities in monoamine and tryptophan metabolites in cerebrospinal fluid from patients with neurological disorders
Author(s) -
Baig S.,
Halawa I.,
Qureshi G. A.
Publication year - 1991
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130050304
Subject(s) - chemistry , chromatography , cerebrospinal fluid , monoamine neurotransmitter , tryptophan , acetic acid , serotonin , high performance liquid chromatography , medicine , biochemistry , amino acid , receptor
In this study we report the levels of 3‐methoxy‐4‐hydroxyphenylglycol, 3,4‐dihydroxyphenylacetic acid, homovallinic acid, tryptophan, 5‐hydroxyindole‐3‐acetic acid and serotonin in lumbar cerebrospinal fluid (CSF) from patients with multiple sclerosis, cerebrovascular disease and muscular tension headache the latter, as healthy controls. The separation of these substances was performed on a reversed phase column by ion pair high performance liquid chromatography and detection was made by a glassy carbon electrode set at +900 mV vs Ag + /AgCI. The whole separation was achieved within 25 min. Concentrations of all substances (10‐1000 pmole/L) were linearly proportional to areas obtained. The system is sensitive, stable and reproducible. The significance of CSF levels of these metabolites from patient groups compared with healthy controls are discussed.