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Separation of proteins by consecutive sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis and reversed phase high performance liquid chromatography
Author(s) -
Kaplan Batia,
Pras Mordechai
Publication year - 1991
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130050209
Subject(s) - chromatography , chemistry , sodium dodecyl sulfate , high performance liquid chromatography , polyacrylamide gel electrophoresis , gel electrophoresis , elution , gel electrophoresis of proteins , acetonitrile , two dimensional gel electrophoresis , sodium , biochemistry , organic chemistry , proteomics , gene , enzyme
A procedure for the preparative separation of proteins was developed by using consecutively sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (SDS‐PAGE) and reversed phase high performance liquid chromatography (HPLC). The proteins were separated by SDS‐PAGE and afterwards extracted from the gel. The extracted proteins were separated from SDS and other small molecular weight contaminants on a Fractogel TSK HW‐40 (F) column in acidic aqueous acetonitrile. The proteins eluted from the Fractogel column were fractionated by HPLC. The identity and purity of the recovered proteins was confirmed by SDS‐PAGE analysis.