Premium
Separation and quantification of histamine and N τ ‐methylhistamine in brain extracts
Author(s) -
Pastó Manel,
Sabria Josefa
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040608
Subject(s) - chemistry , perchloric acid , chromatography , histamine , methanol , elution , sodium , high performance liquid chromatography , inorganic chemistry , pharmacology , organic chemistry , medicine
Histamine and its N τ ‐methyl derivative can be separated from perchloric acid extracts of rat brain by high performance liquid chromatography on a C 18 column under isocratic conditions eluting with 0.1 M sodium phosphate buffer containing 0.19 mM sodium dodecyl sulphate and 25% methanol. Using electrochemical detection, histamine and N τ ‐methylhistamine can be detected at levels of less than 40 pg/μL tissue extract (<1 pmol). The retention times for histamine and N τ ‐methylhistamine were 15 min and 23 min, respectively, at a flow rate of 1.2 mL/min, and both compounds eluted as acceptably sharp peaks. The concentrations of histamine and N τ ‐methylhistamine in brain from seven‐day‐old rats were found to be very similar to those obtained by other analytical procedures.