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Identification of 17‐hydroxyprogesterone and other steroid hormones in saliva from a normal child and patients with congenital adrenal hyperplasia by plasmaspray liquid chromatography/mass spectrometry
Author(s) -
Shindo Noriko,
Yamauchi Noriko,
Murayama Kimie,
Fairbrother Ann,
Krolik Stefan
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040413
Subject(s) - congenital adrenal hyperplasia , chemistry , hydroxyprogesterone , pregnenolone , aldosterone , steroid , chromatography , saliva , endocrinology , testosterone (patch) , medicine , steroid hormone , mineralocorticoid , hormone , mass spectrometry , biochemistry
Abstract A very sensitive, selective and simultaneous method for the analysis of salivary steroid hormones was examined by discharge‐assisted thermospray liquid chromatography mass spectrometry (plasmaspray LC/MS). Plasmaspray LC/MS gave [M + M] + or [MH — H 2 O] + as a predominant ion in most of the steroids in this work and in some cases fragment ions were also observed. Eight salivary steroid hormones, pregnenolone, 17‐hydroxypregnenolone, progesterone, 17‐hydroxyprogesterone, 11‐deoxycortisol, cortisol, aldosterone, and testosterone were identified within 10 minutes using the selected ion monitoring technique in conjunction with plasmaspray LC/MS. Progesterone, 17‐hydroxyprogesterone, cortisol, aldosterone and testosterone were detected in 1 mL of saliva from a normal child and two patients with congenital adrenal hyperplasia.