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Isocratic reversed phase high performance liquid chromatography determination of twelve natural corticosteroids in serum with on‐line ultraviolet and fluorescence detection
Author(s) -
Wei Jiqing,
Wei Jilu,
Zhou Xianteng,
Cheng Jiping
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040410
Subject(s) - chemistry , chromatography , estrone , high performance liquid chromatography , estriol , cortisone , detection limit , derivatization , endocrinology , medicine , hormone , biochemistry
An isocratic reversed phase high performance liquid chromatography procedure utilizing ultraviolet and fluorescence detectors linked in series is described for the analysis of cortisone (E), cortisol (F), corticosterone (B), 11‐deoxycortisol (S), 11‐deoxycorticosterone (DOC), androstenedione (A), testosterone (T), 17‐hydroxyprogesterone (17‐OHP), progesterone (P), estriol, estradiol, estrone, prednisone acetate and dexamethasone acetate in serum. Serum specimens were extracted with ethyl ether. The optimized mobile phase was methanol + tetrahydrofuran + water 26:18:56, v/v/v). A Shim‐pack ODS column was used. The recoveries were 80 to 103%. lntra‐ and inter‐day coefficient of variance were less than 8%. The detection limit is 0.5 pmol per injection volume for estriol, estradiol, E, F and B; 1 pmol for S, A, DOC and estrone; 2 pmol for T and 17‐OHP; and 4 pmol for P. Serum from normal subjects and patients with congenital adrenal hyperplasia due to 21‐ or l7‐hydroxylase deficiency were measured, as well as samples of maternal and umbilical cord serum.