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Chiral separation and determination of propranolol enantiomers in rat or mouse blood and tissue by column switching high performance liquid chromatography with ovomucoid bonded stationary phase
Author(s) -
Tamai Gen,
Edani Masami,
Imai Hideo
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040409
Subject(s) - chemistry , chromatography , enantiomer , resolution (logic) , elution , high performance liquid chromatography , reproducibility , detection limit , analytical chemistry (journal) , stereochemistry , artificial intelligence , computer science
Resolution of propranolol (PL) enantiomers in biological samples was accomplished by column switching high performance liquid chromatography using a short precolumn and an analytical column of ovomucoid chiral phase. Plasma, whole blood or tissue homogenate sample was directly injected into the precolumn, and PL was adsorbed on Butyl Toyopearl 650‐M. After column switching, the PL was backflushed and transferred to the analytical column (Ultron ES‐OVM) by the eluant. Fluorometric detection was carried out at λ ex = 297 nm and λ em = 340 nm with a detection limit of 0.5 pmol (signal to noise ratio = 2). The recovery 98.8–103%), reproducibility (coefficient of variance less than 3%) and enantiomer resolution (separation factor 1.15) were satisfactory using as eluant 50 mM sodium dihydrogenphosphate (pH 4.6) containing 12% ethanol. The time course of elimination of PL enantiomers in rat or mouse blood and tissues was also studied.