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High performance liquid chromatography determination of doxorubicin and daunorubicin in plasma using UV detection and column switching
Author(s) -
Mikan A.,
Lanao J. Martinez,
Lopez F. Gonzalez,
Hurle A. DominguezGil
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040408
Subject(s) - daunorubicin , chemistry , chromatography , doxorubicin , detection limit , plasma , coefficient of variation , high performance liquid chromatography , chemotherapy , medicine , physics , surgery , quantum mechanics
A method for the determination of doxorubicin and daunorubicin in plasma is described. The plasma is injected directly into a loop column and then washed with water. After switching the injection valve, the sample is separated on a phenyl column using detection at 254 nm. The detection limit is 10 ng/mL, the coefficient of variation is 7% for 100 ng/mL of doxorubicin and 4% for 200 ng/mL of daunorubicin.