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Determination of 6‐mercaptopurine and its metabolites in plasma or serum by high performance liquid chromatography
Author(s) -
Sahnoun Z.,
SerreDebeauvais F.,
Lang J.,
Faucon G.,
Gavend M.
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040405
Subject(s) - chemistry , chromatography , mercaptopurine , plasma , pharmacology , physics , quantum mechanics , medicine
A sensitive and accurate reversed phase liquid chromatographic assay was developed for the determination of 6‐mercaptopurine (6MP) (the active metabolite of azathioprine) in human plasma. The assay involved extraction into acetonitrile and dichloromethane from plasma pretreated with 0.038 M of dithiothreitol solution. The residue was analyzed by isocratic chromatography on a C 18 analytical column with UV detection at 326 nm. The average extraction recovery of 6MP was 85%. The method has been applied successfully to the determination of 6MP and its metabolites in pharmacokinetic studies.