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Chemiluminescence high performance liquid chromatography of corticosteroids using lucigenin as post‐column reagent
Author(s) -
Takeda Motoko,
Maeda Masako,
Tsuji Akio
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040309
Subject(s) - chemistry , lucigenin , chromatography , chemiluminescence , reagent , column chromatography , methanol , high performance liquid chromatography , organic chemistry , enzyme , superoxide
Abstract A chemiluminescence high performance liquid chromatographic method for the determination of corticosteroids and tetrahydrocorticosteroids has been developed. Corticosteroids and their metabolites extracted from urine samples were separated using an ODS column and a mixture of methanol + water + 0.01 M sodium acetate solution (70:30:5) as eluent. The eluent from the column was mixed with the chemiluminescent solution containing lucigenin and Triton X‐100 and a 0.28 M KOH solution by pumps and monitored by a chemiluminescence detector. No interference was encountered and the method is both precise and reproducible.