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Evaluation of pyrimido[5,4‐ d ]pyrimidine derivatives as peroxyoxalate chemiluminescence reagents using a flow injection system
Author(s) -
Nakashima Kenichiro,
Maki Kouichi,
Akiyama Shuzo,
Imai Kazuhiro
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040306
Subject(s) - peroxyoxalate , chemiluminescence , chemistry , perylene , reagent , hydrogen peroxide , triethylamine , fluorophore , oxalate , fluorescence , pyrimidine , chromatography , rhodamine b , photochemistry , organic chemistry , catalysis , stereochemistry , molecule , photocatalysis , physics , quantum mechanics
Eighteen kinds of pyrimido[5,4‐ d ]pyrimidines together with several commercially available fluorescent compounds such as perylene, Rhodamine B, etc., were evaluated as the reagents for a peroxyoxalate chemiluminescence (CL) detection system by using a flow injection method. The peroxyoxalate CL reaction employed consists of bis(2,4,6‐trichlorophenyl)oxalate, hydrogen peroxide, triethylamine, and a fluorophore. Under the conditions used, 2,6‐bis[di‐(2‐hydroxyethyl)amino]‐4,8‐dipiperidinopyrimido[5,4‐ d ]pyrimridine (Dipyridamole) and 2,4,6,8‐tetrathiomorpholinopyrimido[5,4‐ d ]pyrimidine (1i) gave very intense chemiluminescence intensities which were larger than those of any other commercially available fluorescent compounds tested (e.g., 10 times larger than that of perylene).