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Removal of sodium dodecyl sulphate from proteins isolated by sodium dodecyl sulphate polyacrylamide gel electrophoresis
Author(s) -
Kaplan Batia,
Pras Mordechai
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040212
Subject(s) - chemistry , chromatography , sodium dodecyl sulfate , coomassie brilliant blue , polyacrylamide gel electrophoresis , sodium , gel electrophoresis , gel permeation chromatography , tris , acetonitrile , gel electrophoresis of proteins , electrophoresis , biochemistry , staining , organic chemistry , polymer , medicine , pathology , enzyme
Abstract A procedure is described for the removal of sodium dodecyl sulphate (SDS) from proteins isolated by SDS‐polyacrylamide gel electrophoresis (SDS‐PAGE). The proteins separated by SDS‐PAGE were stained with Coomassie Blue and extracted with Tris‐HCI buffer containing SDS. The obtained extracts were subjected to gel permeation chromatography in an acidic aqueous acetonitrile solution. The procedure allows purification of the isolated proteins not only from SDS, but also from Coomassie Blue, buffer salts and other small molecular weight contaminants.