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A simple, sensitive liquid chromatographic method for quantitation of D ‐galactose and D ‐glucose in blood plasma
Author(s) -
Griffin Brenda W.,
Williams Gary W.
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040211
Subject(s) - chemistry , chromatography , galactose , monosaccharide , elution , biochemistry
The concentrations of D‐glucose and D‐galactose in plasma of galactosemic rats were quantitatively measured by a liquid chromatographic method, based on retention of the weakly ionized monosaccharides by an anion exchange column under alkaline conditions, elution with 9 mmol/L NaOH, and electrochemical detection. This method is both simple and sensitive, since very dilute plasma samples can be directly analysed.