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A new method for the quantitation of aflatoxin M 1 in urine by high performance liquid chromatography and its application to the etiologic study of hepatoma
Author(s) -
Liu Zhonghe,
Tu Wensheng,
Li Danrong,
Li Yuandong,
Xie Chonghu,
Yang Yinzhong,
Qin Bingbing
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040210
Subject(s) - chromatography , chemistry , aflatoxin , urine , high performance liquid chromatography , chloroform , detection limit , urine sample , derivative (finance) , sample preparation , biochemistry , food science , financial economics , economics
A high performance liquid chromatographic (HPLC) method for the analysis of aflotoxin M 1 (AFM 1 ) in urine is described. Urine samples were treated with saturated lead acetate and AFM 1 was extracted with chloroform. After washing with water to remove impurities the compound was derivatized with trifluoracetic acid and the AFM 1 derivative was analyzed quantitatively by HPLC. The sample pretreatment is simple and more selective. A good line correlation between AFM 1 peak height and its concentration was obtained when AFM 1 content was in the range of 50–400 pg. The ratio of recovery was 87.42%. Sensitivity is 0.01 ppb. The method is applicable to trace analysis. Results in urine of residents who live in the high/low liver cancer incidence area in Fushui county were the same as that of previous epidemiological investigation.