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Rapid detection of extended ganglio‐series gangliosides with terminal GalNAcβl‐4Gal sequence on high performance thin layer chromatography plates
Author(s) -
Müthing Johannes,
Ziehr Holger
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040207
Subject(s) - monoclonal antibody , chemistry , neuraminidase , affinity chromatography , antibody , glycolipid , antigen , microbiology and biotechnology , chromatography , biochemistry , biology , immunology , enzyme
The mouse monoclonal antibody 2D4, which recognizes the terminal GalNAcβ1‐4Gal‐disaccharide of GgOse 3 Cer and GgOse 5 Cer, was used for the detection of ganglio‐series gangliosides. The method involves separation of gangliosides on thin layer chromatography plates, followed by silica gel fixation, Arthrobacter ureafaciens neuraminidase treatment and final immunostaining of desialylated gangliosides with the monoclonal antibody 2D4. Both neuraminidase and the hybridoma 2D4 producing the specific monoclonal antibody are commercially available and therefore accessible to all researchers working in this field. Gangliosides from mouse T lymphocytes and the mouse T cell lymphoma YAC‐1 have been used as examples. This technique may be used for fast screening of gangliosides with the GgOse 5 Cer core structure which have been described as T cell markers, antigens in human neuronal disease and receptors for certain pathogenic bacteria.