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Optimization of an Isocratic Reversed Phase Liquid Chromatographic System for the Separation of Fourteen Steroids Using Factorial Design and Computer Simulation
Author(s) -
Wei JiQing,
Wei JiLu,
Zhou XianTeng
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040106
Subject(s) - chemistry , chromatography , estrone , androstenedione , high performance liquid chromatography , factorial experiment , estriol , resolution (logic) , hydroxycorticosteroids , androgen , hormone , biochemistry , statistics , mathematics , artificial intelligence , computer science , urine
An optimization strategy for an isocratic reversed phase high performance liquid chromatographic system (RP‐HPLC) is described. Factorial design and a computer program are used to predict the retention time and resolution of fourteen steroids. An optimized rapid (<25 min) isocratic RP‐HPLC system for the satisfactory separation of cortisone, cortisol, corticosterone, 11‐deoxycortisol, 11‐deoxycorticosterone, 17 α ‐hydroxyprogesterone, progesterone, androstenedione, testosterone, estrone, estradiol, estriol, prednisone acetate and dexamethasone acetate has been developed using this strategy through eight experiments.