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Rapid quantitative determination of epirubicin and its metabolites in plasma using high performance liquid chromatography and fluorescence detection
Author(s) -
Dine T.,
Brunet C.,
Luyckx M.,
Cazin M.,
Gosselin P.,
Cazin J. L.
Publication year - 1990
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130040103
Subject(s) - chemistry , chromatography , epirubicin , high performance liquid chromatography , daunorubicin , fluorescence , pharmacokinetics , quantitative analysis (chemistry) , cartridge , cancer , pharmacology , leukemia , breast cancer , medicine , mechanical engineering , physics , quantum mechanics , engineering
Abstract A rapid sensitive and selective isocratic technique was developed for the analysis of plasma epirubicin and three of its known fluorescent metabolites epirubicinol, 4′‐ O ‐β‐D‐glucuronyl‐4′‐epidoxorubicin and 4′‐ O ‐β‐D‐glucuronyl 1,3‐dihydro‐4′‐epidoxorubicin, with daunorubicin as an internal standard, by using high performance liquid chromatography (HPLC) with fluorescence detection and a ‘Hypersil ODS’ column. The drugs were easily and efficiently extracted with a Sep‐Pak C 18 cartridge and the mean recoveries were greater than 85%. Intraassay and Interassay coefficients of variation (plasma samples) were better than 8.25%. An example of pharmacokinetic study obtained in a cancer patient after intravenous injection of epirubicine is described.