A rapid method for isolation of human hemoglobin benzo[ a ]pyrene diol epoxide derived adducts using high performance liquid chromatography | Zendy

Naylor S. | Zendy; Gan L.S. | Zendy; Day B. W. | Zendy; Skipper P. L. | Zendy; Tannenbaum S. R. | Zendy

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A rapid method for isolation of human hemoglobin benzo[ a ]pyrene diol epoxide derived adducts using high performance liquid chromatography

Author(s) -

Naylor S.,

Gan L.S.,

Day B. W.,

Skipper P. L.,

Tannenbaum S. R.

Publication year - 1989

Publication title -

biomedical chromatography

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.4

H-Index - 65

eISSN - 1099-0801

pISSN - 0269-3879

DOI - 10.1002/bmc.1130030608

Subject(s) - chemistry , chromatography , adduct , high performance liquid chromatography , pyrene , epoxide , diol , benzo(a)pyrene , hemoglobin , organic chemistry , catalysis

A method is described to isolate rapidly human hemoglobin‐benzo[ a ]pyrene diol epoxide adducts. A combination of 300 Å pore size C 4 reversed phase HPLC to effect separation of adducted protein from native protein, and μ‐bore C 18 reversed phase HPLC to isolate and partially characterize proteolytic peptide adducts (by UV), was used.

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