Premium
A rapid method for isolation of human hemoglobin benzo[ a ]pyrene diol epoxide derived adducts using high performance liquid chromatography
Author(s) -
Naylor S.,
Gan L.S.,
Day B. W.,
Skipper P. L.,
Tannenbaum S. R.
Publication year - 1989
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130030608
Subject(s) - chemistry , chromatography , adduct , high performance liquid chromatography , pyrene , epoxide , diol , benzo(a)pyrene , hemoglobin , organic chemistry , catalysis
Abstract A method is described to isolate rapidly human hemoglobin‐benzo[ a ]pyrene diol epoxide adducts. A combination of 300 Å pore size C 4 reversed phase HPLC to effect separation of adducted protein from native protein, and μ‐bore C 18 reversed phase HPLC to isolate and partially characterize proteolytic peptide adducts (by UV), was used.