Premium
Simultaneous analysis of furosemide and bumetanide in horse plasma using high performance liquid chromatography
Author(s) -
Singh Ashok K.,
McArdle Camille,
Gordon Brad,
Ashraf M.,
Granley K.
Publication year - 1989
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130030607
Subject(s) - chemistry , bumetanide , furosemide , chromatography , horse , plasma , high performance liquid chromatography , membrane , ion transporter , biochemistry , paleontology , physics , organic chemistry , quantum mechanics , biology
A high performance liquid chromatographic method is described for the simultaneous determination of furosemide and bumetanide in horse plasma. The C 8 (3 μm) reversed phase column (4.8 × 150 mm) provided clear separation of furosemide and bumetanide with other components present in the horse plasma. The detection limit for both the drugs was 10 ng/mL. Both drugs were stable in plasma (at natural or acidic pH) for up to 24 h. The method is sufficiently sensitive to detect furosemide levels in plasma obtained from horses receiving a therapeutic dose of furosemide.