Premium
Multidimensional chromatographic separation of estrogen receptors
Author(s) -
Raubenheimer J.,
van der Walt L. A.
Publication year - 1989
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130030606
Subject(s) - chemistry , estrogen receptor , chromatography , receptor , estrogen , density gradient , high performance liquid chromatography , size exclusion chromatography , sucrose , gene isoform , biochemistry , endocrinology , medicine , cancer , breast cancer , biology , physics , quantum mechanics , enzyme , gene
Analyses of estrogen and progesterone receptors in biopsies of breast carcinoma play a vital role in the selection of patients likely to respond to hormone manipulation. Sucrose density gradient centrifugation has been the reference method in the determination of estrogen receptors in human breast carcinoma cytosols. To reduce assay time and circumvent prolonged manipulation of labile receptor preparations, high performance liquid chromatography techniques in the size‐exclusion and ion‐exchange modes were compared as potential alternate methods for the rapid separation of receptor isoforms. Multidimensional analyses were performed by reapplying estrogen receptor isoforms obtained from high performance size‐exclusion and ion‐exchange chromatography to sucrose density gradients and vice versa. This confirmed that the estrogen‐binding components identified by high performance liquid chromatography appear to correspond to estrogen receptor species from sucrose density gradients.