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Reversed phase high performance liquid chromatography of glycopeptides
Author(s) -
Wang Tsonghui,
Chen Tsefang,
Barofsky Douglas F.
Publication year - 1989
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130030603
Subject(s) - chemistry , chromatography , glycopeptide , hydrophilic interaction chromatography , high performance liquid chromatography , mass spectrometry , hydrolysis , fast atom bombardment , reversed phase chromatography , ovalbumin , ion chromatography , phase (matter) , gel permeation chromatography , chromatography column , organic chemistry , biochemistry , polymer , immune system , immunology , biology , antibiotics
A practical procedure for isolating and purifying glycopeptides is described, viz. enzymatic hydrolysis—gel permeation chromatography—ion exchange chromatography—reversed phase HPLC. Using this procedure 28 glycopeptides from hen ovalbumin have been isolated some of which hitherto have not been identified. Water was a suitable mobile phase for preparing pure glycopeptides, and control of column temperature was important for good separations and reproducible retention times. Structural confirmation was by fast atom bombardment mass spectrometry.