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High performance liquid chromatography determination of cyanide in urine by pre‐column fluorescence derivatization
Author(s) -
Sano Akira,
Takezawa Masaaki,
Takitani Shoji
Publication year - 1989
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130030507
Subject(s) - chemistry , chromatography , cyanide , derivatization , urine , detection limit , high performance liquid chromatography , dilution , biochemistry , organic chemistry , physics , thermodynamics
A method for the determination of cyanide in human urine has been developed. The method is based on the reaction of cyanide with 2,3‐naphthalenedialdehyde and taurine to give a fluorescent product for reversed‐phase HPLC separation and fluorometric detection. After centrifugation followed by dilution of urine samples, the specimens could be analysed directly by this method. The recovery of cyanide added to urine at concentration levels of 50 – 1000 pmol/mL was 85 – 96%. The detection limit of cyanide was 30 pmol/mL in urine. The method was successfully applied to the analysis of urine from smokers and nonsmokers. The mean concentrations of cyanide were found to be 215 pmol/mL for the former and 84 pmol/mL for the latter.