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Rapid and sensitive determination of β‐phenylethylamine in animal brains by high performance liquid chromatography with fluorometric detection
Author(s) -
Taga Chiaki,
Tsuji Motohiro,
Nakajima Teruo
Publication year - 1989
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130030306
Subject(s) - chemistry , chromatography , amberlite , elution , high performance liquid chromatography , organic chemistry , adsorption
A reversed phase HPLC method with fluorometric detection for the analysis of β‐phenylethylamine has been developed using p ‐methoxyphenylethylamine as an internal standard. Two columns, containing 200 μL of Dowex 50‐X8 and Amberlite CG‐50 respectively, were used to prepare a fraction containing β‐phenylethylamine. The recoveries of β‐phenylethylamine and p ‐methoxyphenylethylamine were 53.9 ± 9.4% and 68.1 ± 12.4%, respectively, and elution profile of p ‐methoxyphenylethylamine was sufficiently well correlated with that of β‐phenylethylamine. Regional distributions of β‐phenylethylamine in rat and mouse brains were determined. The highest concentrations were found in hypothalamus and hippocampus in both animals.