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Determination of urinary 5‐hydroxy‐3‐indoleacetic acid by an automated HPLC method
Author(s) -
Koel Marlies,
Nebinger Peter
Publication year - 1989
Publication title -
biomedical chromatography
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.4
H-Index - 65
eISSN - 1099-0801
pISSN - 0269-3879
DOI - 10.1002/bmc.1130030305
Subject(s) - chemistry , chromatography , gradient elution , detection limit , high performance liquid chromatography , elution , urine , quantitative analysis (chemistry) , biochemistry
A HPLC method for the quantitative determination of 5‐hydroxy‐3‐indoleacetic acid (5‐HIAA) in urine is described. The method is based on ion‐pair chromatography, reversed phase (RP) column material and specific fluorimetric detection at 300 nm and 355 nm. Sample preparation and gradient elution were avoided by using a column‐switching technique. The sensitivity of the assay was excellent for clinical routine analysis, with a detection limit of 0.2 mg/L 5‐HIAA. No endogenous or exogenous interference problems arose. Intra‐ and interassay precision was good, with observed coefficients of variation of 1.5 to 2.6% and 2.1%, respectively. Recoveries were 93 to 98%. The system described can be used for clinical diagnosis and therapy follow‐up of carcinoid tumors. It has been running for over a year without disturbances and with a minimum of technical attendance.